Acta Parasitologica Homepage
Tables of Contents
Volume 44 Tables of Contents

Acta Parasitologica, Vol.44, No. 1, 1999, 81-83
Mieszczanek Juliusz, Wedrychowicz Halina - Differentiation of two dog hookworm species using PCR.

Department of Parasitology, Faculty of Veterinary Medicine, Warsaw Agricultural University, Grochowska 272, 03-849 Warszawa, Poland

Reliable determination of hookworm nematode parasite species from dogs can be carried out post-mortem by microscopical examination of the buccal cavity of adult worms isolated from the host small intestine. In order to allow a proper diagnosis of the hookworm infection in a living host, we developed a polymerase chain reaction (PCR). Using DNA extracted from hookworm eggs and an oligonucleotide set designed on the basis of the DNA sequence of a cysteine proteinase AcCP1 gene, Ancylostoma caninum and Uncinaria stenocephala DNA can be discriminated from each other.

KEY WORDS: Uncinaria stenocephala, Ancylostoma caninum, hookworms, PCR
Page compiled by Aleksander H.Kedra. Last modification: 05-10-1999