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Acta Parasitologica, Vol.44, No. 2, 1999, 147-148
Tkach Vasyl (1), Pawlowski Jan (2) - A new method of DNA extraction from the ethanol-fixed parasitic worms.

(1) Department of Parasitology, I. I. Shmalghausen Institute of Zoology, Ukrainian Academy of Sciences, 15 Bogdan Khmelnitski Street, Kiev-30, MSP, 252601 Ukraine; (2) Department of Zoology and Animal Biology, University of Geneva, 154 route de Malagnou, CH-1224, Chjne-Bougeries/Geneva, Switzerland

A protocol of the new method of DNA extraction from the ethanol fixed parasitic worms is described. The method is based on the use of the guanidine thiocyanate extraction buffer after evaporation of the ethanol from the tissues by drying. The method has been successfully used on several groups of parasitic Plathyhelminthes and provided higher DNA yield than a conventional phenol-chloroform extraction.

KEY WORDS: parasitic worms, DNA extraction, PCR
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