Tkach Vasyl (1), Pawlowski Jan (2)
- A new method of DNA extraction from the ethanol-fixed parasitic worms.
(1) Department of Parasitology, I. I. Shmalghausen Institute of Zoology,
Ukrainian Academy of Sciences, 15 Bogdan Khmelnitski Street,
Kiev-30, MSP, 252601 Ukraine; (2) Department of Zoology and Animal
Biology, University of Geneva, 154 route de Malagnou, CH-1224,
A protocol of the new method of DNA extraction from the ethanol fixed
worms is described. The method is based on the use of the guanidine
buffer after evaporation of the ethanol from the tissues by drying. The
method has been
successfully used on several groups of parasitic Plathyhelminthes and
provided higher DNA
yield than a conventional phenol-chloroform extraction.
parasitic worms, DNA extraction, PCR
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