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A total of 31 European bison (Bison bonasus L.) from closed Polish breeding facilities were examined. Eleven ectoparasites were detected from Insecta (Bisonicola sedecimdecembrii, Lipoptena cervi, Melophagus ovinus) and Acari (Ixodes ricinus, I. persulcatus, I. hexagonus, Dermacentor reticulatus, Chorioptes bovis, Psoroptes ovis, Demodex bisonianus, D. bovis). The predominant parasites were I. ricinus, D. bisonianus and B. sedecimdecembrii.