Volume 48/Number 2/Abstract 11
Acta Parasitologica Homepage
Tables of Contents
Volume 48 Tables of Contents

Acta Parasitologica, Vol.48, No. 2, 2003, 135-137
Lucinda Paulsen and Stephanus N. Venter* - Genotyping of Cryptosporidium parvum isolates obtained from humans in South Africa

Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria, 0002, South Africa
*Corresponding author: snventer@postino.up.ac.za

Cryptosporidium parvum is endemic to South Africa. In order to provide direction for prevention strategies, information on the dominant genotype involved in human infections is required. A combination of diethyl ether extraction, sodium chloride flotation and immuno-magnetic separation (IMS) was used for maximum recovery of oocysts from faecal samples. In order to improve the sensitivity of PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene, a nested PCR was developed. RFLP analysis was done on a 553 bp segment obtained from the final nested PCR reaction to distinguish between C. parvum genotype 1 (human exclusive type) and genotype 2 (broad host range). Overall, genotype 2 was detected in 10 samples, whereas genotype 1 was detected in the remaining 40 samples. The high incidence of genotype 1 parasites (80%) implies that direct and indirect human transmission plays a dominant role in the epidemiology of the disease caused by this parasite in the study area.

KEY WORDS: Cryptosporidium parvum , PCR-RFLP, COWP gene, genotypes

Page compiled by M. Bultowicz. Last modification: 05-08-2003