The infection of metacercariae of Posthodiplostomum cuticola (v. Nordmann, 1832), the agent of black-spot disease, was investigated in 0+ juvenile fish from 19 localities in south-east Moravia, Czech Republic. Of the 30 fish species examined, only cyprinids of 12 species were found to be infected. Five species of Leuciscinae, Rutilus rutilus, Leuciscus idus, Scardinius erythrophthalmus, Abramis bjoerkna and Abramis brama, fish with similar behaviour and habitat preference in juvenile period, were the most susceptible fish hosts for P. cuticola metacercariae. Especially high infection parameters were found in intergeneric hybrids of the studied leuciscine species. Environmental conditions were an important factor for parasite infection. The fish from flooded borrow pits, inhabiting lentic water bodies with dense vegetation in the littoral zone, showed high prevalence and abundance of P. cuticola infection contrary to their conspecifics in water reservoirs with steep banks and fish from rivers.