Acta Parasitologica, Vol. 52, No. 3, 2007, 295–297. DOI: 10.2478/s11686-007-0041-0 Katarzyna Goździk* and Władysław Cabaj –
Characterization of the first Polish isolate of Neospora caninum from cattle
W. Stefanski Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818 Warsaw, Poland
*Corresponding author: kasiagoz@twarda.pan.pl
ABSTRACT
Neospora caninum was isolated from the brain of an apparently healthy calf born to a seropositive cow. The calf was killed 12
hrs after birth and homogenate of its brain was inoculated into Vero cells. Neospora-like tachyzoites were detected 66 days later.
The identity of the parasite was confirmed by polymerase chain reaction amplification of N. caninum-specific fragments using
Np21 and Np6 primers. This first Polish isolate of N. caninum was designated NC-PolB1.
KEY WORDS:
Neospora caninum, isolation, PCR, ELISA, Western blot, calf