Volume 52/Number3/Abstract 17
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Acta Parasitologica, Vol. 52, No. 3, 2007, 295–297. DOI: 10.2478/s11686-007-0041-0
Katarzyna Goździk* and Władysław Cabaj – Characterization of the first Polish isolate of Neospora caninum from cattle

W. Stefanski Institute of Parasitology, Polish Academy of Sciences, 51/55 Twarda Street, 00-818 Warsaw, Poland
*Corresponding author: kasiagoz@twarda.pan.pl
Neospora caninum was isolated from the brain of an apparently healthy calf born to a seropositive cow. The calf was killed 12 hrs after birth and homogenate of its brain was inoculated into Vero cells. Neospora-like tachyzoites were detected 66 days later. The identity of the parasite was confirmed by polymerase chain reaction amplification of N. caninum-specific fragments using Np21 and Np6 primers. This first Polish isolate of N. caninum was designated NC-PolB1.
KEY WORDS: Neospora caninum, isolation, PCR, ELISA, Western blot, calf

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